Identification and complete genome sequencing of paramyxoviruses in mallard ducks (Anas platyrhynchos) using random access amplification and next generation sequencing technologies.
Identifieur interne : 000060 ( Main/Exploration ); précédent : 000059; suivant : 000061Identification and complete genome sequencing of paramyxoviruses in mallard ducks (Anas platyrhynchos) using random access amplification and next generation sequencing technologies.
Auteurs : Toon Rosseel [Belgique] ; Bénédicte Lambrecht ; Frank Vandenbussche ; Thierry Van Den Berg ; Steven Van BormSource :
- Virology journal [ 1743-422X ] ; 2011.
Descripteurs français
- KwdFr :
- ARN viral (génétique), Animaux, Avulavirus (), Avulavirus (génétique), Avulavirus (isolement et purification), Belgique, Canards (virologie), Données de séquences moléculaires, Génome viral, Génotype, Infections à avulavirus (médecine vétérinaire), Infections à avulavirus (virologie), Séquençage nucléotidique à haut débit, Sérotypage, Variation génétique.
- MESH :
- génétique : ARN viral, Avulavirus.
- isolement et purification : Avulavirus.
- médecine vétérinaire : Infections à avulavirus.
- virologie : Canards, Infections à avulavirus.
- Animaux, Avulavirus, Belgique, Données de séquences moléculaires, Génome viral, Génotype, Séquençage nucléotidique à haut débit, Sérotypage, Variation génétique.
- Wicri :
- geographic : Belgique.
English descriptors
- KwdEn :
- Animals, Avulavirus (classification), Avulavirus (genetics), Avulavirus (isolation & purification), Avulavirus Infections (veterinary), Avulavirus Infections (virology), Belgium, Ducks (virology), Genetic Variation, Genome, Viral, Genotype, High-Throughput Nucleotide Sequencing, Molecular Sequence Data, RNA, Viral (genetics), Serotyping.
- MESH :
- chemical , genetics : RNA, Viral.
- geographic : Belgium.
- classification : Avulavirus.
- genetics : Avulavirus.
- isolation & purification : Avulavirus.
- veterinary : Avulavirus Infections.
- virology : Avulavirus Infections, Ducks.
- Animals, Genetic Variation, Genome, Viral, Genotype, High-Throughput Nucleotide Sequencing, Molecular Sequence Data, Serotyping.
Abstract
BACKGROUND
During a wildlife screening program for avian influenza A viruses (AIV) and avian paramyxoviruses (APMV) in Belgium, we isolated two hemagglutinating agents from pools of cloacal swabs of wild mallards (Anas platyrhynchos) caught in a single sampling site at two different times. AIV and APMV1 were excluded using hemagglutination inhibition (HI) testing and specific real-time RT-PCR tests.
METHODS
To refine the virological identification of APMV2-10 realized by HI subtyping tests and in lack of validated molecular tests for APMV2-10, random access amplification was used in combination with next generation sequencing for the sequence independent identification of the viruses and the determination of their genomes.
RESULTS
Three different APMVs were identified. From one pooled sample, the complete genome sequence (15054 nucleotides) of an APMV4 was assembled from the random sequences. From the second pooled sample, the nearly complete genome sequence of an APMV6 (genome size of 16236 nucleotides) was determined, as well as a partial sequence for an APMV4. This APMV4 was closely related but not identical to the APMV4 isolated from the first sample. Although a cross-reactivity with other APMV subtypes did not allow formal identification, the HI subtyping revealed APMV4 and APMV6 in the respective pooled samples but failed to identify the co-infecting APMV4 in the APMV6 infected pool.
CONCLUSIONS
These data further contribute to the knowledge about the genetic diversity within the serotypes APMV4 and 6, and confirm the limited sensitivity of the HI subtyping test. Moreover, this study demonstrates the value of a random access nucleic acid amplification method in combination with massive parallel sequencing. Using only a moderate and economical sequencing effort, the characterization and full genome sequencing of APMVs can be obtained, including the identification of viruses in mixed infections.
DOI: 10.1186/1743-422X-8-463
PubMed: 21978491
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Identification and complete genome sequencing of paramyxoviruses in mallard ducks (Anas platyrhynchos) using random access amplification and next generation sequencing technologies.</title><author><name sortKey="Rosseel, Toon" sort="Rosseel, Toon" uniqKey="Rosseel T" first="Toon" last="Rosseel">Toon Rosseel</name><affiliation wicri:level="1"><nlm:affiliation>Operational Directorate of Virology, Veterinary and Agrochemical Research Center, Ukkel, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Operational Directorate of Virology, Veterinary and Agrochemical Research Center, Ukkel</wicri:regionArea><wicri:noRegion>Ukkel</wicri:noRegion></affiliation></author><author><name sortKey="Lambrecht, Benedicte" sort="Lambrecht, Benedicte" uniqKey="Lambrecht B" first="Bénédicte" last="Lambrecht">Bénédicte Lambrecht</name></author><author><name sortKey="Vandenbussche, Frank" sort="Vandenbussche, Frank" uniqKey="Vandenbussche F" first="Frank" last="Vandenbussche">Frank Vandenbussche</name></author><author><name sortKey="Van Den Berg, Thierry" sort="Van Den Berg, Thierry" uniqKey="Van Den Berg T" first="Thierry" last="Van Den Berg">Thierry Van Den Berg</name></author><author><name sortKey="Van Borm, Steven" sort="Van Borm, Steven" uniqKey="Van Borm S" first="Steven" last="Van Borm">Steven Van Borm</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:21978491</idno><idno type="pmid">21978491</idno><idno type="doi">10.1186/1743-422X-8-463</idno><idno type="wicri:Area/Main/Corpus">000062</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000062</idno><idno type="wicri:Area/Main/Curation">000062</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000062</idno><idno type="wicri:Area/Main/Exploration">000062</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Identification and complete genome sequencing of paramyxoviruses in mallard ducks (Anas platyrhynchos) using random access amplification and next generation sequencing technologies.</title><author><name sortKey="Rosseel, Toon" sort="Rosseel, Toon" uniqKey="Rosseel T" first="Toon" last="Rosseel">Toon Rosseel</name><affiliation wicri:level="1"><nlm:affiliation>Operational Directorate of Virology, Veterinary and Agrochemical Research Center, Ukkel, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Operational Directorate of Virology, Veterinary and Agrochemical Research Center, Ukkel</wicri:regionArea><wicri:noRegion>Ukkel</wicri:noRegion></affiliation></author><author><name sortKey="Lambrecht, Benedicte" sort="Lambrecht, Benedicte" uniqKey="Lambrecht B" first="Bénédicte" last="Lambrecht">Bénédicte Lambrecht</name></author><author><name sortKey="Vandenbussche, Frank" sort="Vandenbussche, Frank" uniqKey="Vandenbussche F" first="Frank" last="Vandenbussche">Frank Vandenbussche</name></author><author><name sortKey="Van Den Berg, Thierry" sort="Van Den Berg, Thierry" uniqKey="Van Den Berg T" first="Thierry" last="Van Den Berg">Thierry Van Den Berg</name></author><author><name sortKey="Van Borm, Steven" sort="Van Borm, Steven" uniqKey="Van Borm S" first="Steven" last="Van Borm">Steven Van Borm</name></author></analytic><series><title level="j">Virology journal</title><idno type="eISSN">1743-422X</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Avulavirus (classification)</term><term>Avulavirus (genetics)</term><term>Avulavirus (isolation & purification)</term><term>Avulavirus Infections (veterinary)</term><term>Avulavirus Infections (virology)</term><term>Belgium</term><term>Ducks (virology)</term><term>Genetic Variation</term><term>Genome, Viral</term><term>Genotype</term><term>High-Throughput Nucleotide Sequencing</term><term>Molecular Sequence Data</term><term>RNA, Viral (genetics)</term><term>Serotyping</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (génétique)</term><term>Animaux</term><term>Avulavirus ()</term><term>Avulavirus (génétique)</term><term>Avulavirus (isolement et purification)</term><term>Belgique</term><term>Canards (virologie)</term><term>Données de séquences moléculaires</term><term>Génome viral</term><term>Génotype</term><term>Infections à avulavirus (médecine vétérinaire)</term><term>Infections à avulavirus (virologie)</term><term>Séquençage nucléotidique à haut débit</term><term>Sérotypage</term><term>Variation génétique</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Belgium</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Avulavirus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Avulavirus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN viral</term><term>Avulavirus</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Avulavirus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Avulavirus</term></keywords><keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Infections à avulavirus</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Avulavirus Infections</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Canards</term><term>Infections à avulavirus</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Avulavirus Infections</term><term>Ducks</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Genetic Variation</term><term>Genome, Viral</term><term>Genotype</term><term>High-Throughput Nucleotide Sequencing</term><term>Molecular Sequence Data</term><term>Serotyping</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Avulavirus</term><term>Belgique</term><term>Données de séquences moléculaires</term><term>Génome viral</term><term>Génotype</term><term>Séquençage nucléotidique à haut débit</term><term>Sérotypage</term><term>Variation génétique</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Belgique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b></p><p>During a wildlife screening program for avian influenza A viruses (AIV) and avian paramyxoviruses (APMV) in Belgium, we isolated two hemagglutinating agents from pools of cloacal swabs of wild mallards (Anas platyrhynchos) caught in a single sampling site at two different times. AIV and APMV1 were excluded using hemagglutination inhibition (HI) testing and specific real-time RT-PCR tests.</p></div><div type="abstract" xml:lang="en"><p><b>METHODS</b></p><p>To refine the virological identification of APMV2-10 realized by HI subtyping tests and in lack of validated molecular tests for APMV2-10, random access amplification was used in combination with next generation sequencing for the sequence independent identification of the viruses and the determination of their genomes.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>Three different APMVs were identified. From one pooled sample, the complete genome sequence (15054 nucleotides) of an APMV4 was assembled from the random sequences. From the second pooled sample, the nearly complete genome sequence of an APMV6 (genome size of 16236 nucleotides) was determined, as well as a partial sequence for an APMV4. This APMV4 was closely related but not identical to the APMV4 isolated from the first sample. Although a cross-reactivity with other APMV subtypes did not allow formal identification, the HI subtyping revealed APMV4 and APMV6 in the respective pooled samples but failed to identify the co-infecting APMV4 in the APMV6 infected pool.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b></p><p>These data further contribute to the knowledge about the genetic diversity within the serotypes APMV4 and 6, and confirm the limited sensitivity of the HI subtyping test. Moreover, this study demonstrates the value of a random access nucleic acid amplification method in combination with massive parallel sequencing. Using only a moderate and economical sequencing effort, the characterization and full genome sequencing of APMVs can be obtained, including the identification of viruses in mixed infections.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21978491</PMID><DateCompleted><Year>2012</Year><Month>02</Month><Day>22</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1743-422X</ISSN><JournalIssue CitedMedium="Internet"><Volume>8</Volume><PubDate><Year>2011</Year><Month>Oct</Month><Day>06</Day></PubDate></JournalIssue><Title>Virology journal</Title><ISOAbbreviation>Virol. J.</ISOAbbreviation></Journal><ArticleTitle>Identification and complete genome sequencing of paramyxoviruses in mallard ducks (Anas platyrhynchos) using random access amplification and next generation sequencing technologies.</ArticleTitle><Pagination><MedlinePgn>463</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1186/1743-422X-8-463</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">During a wildlife screening program for avian influenza A viruses (AIV) and avian paramyxoviruses (APMV) in Belgium, we isolated two hemagglutinating agents from pools of cloacal swabs of wild mallards (Anas platyrhynchos) caught in a single sampling site at two different times. AIV and APMV1 were excluded using hemagglutination inhibition (HI) testing and specific real-time RT-PCR tests.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">To refine the virological identification of APMV2-10 realized by HI subtyping tests and in lack of validated molecular tests for APMV2-10, random access amplification was used in combination with next generation sequencing for the sequence independent identification of the viruses and the determination of their genomes.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">Three different APMVs were identified. From one pooled sample, the complete genome sequence (15054 nucleotides) of an APMV4 was assembled from the random sequences. From the second pooled sample, the nearly complete genome sequence of an APMV6 (genome size of 16236 nucleotides) was determined, as well as a partial sequence for an APMV4. This APMV4 was closely related but not identical to the APMV4 isolated from the first sample. Although a cross-reactivity with other APMV subtypes did not allow formal identification, the HI subtyping revealed APMV4 and APMV6 in the respective pooled samples but failed to identify the co-infecting APMV4 in the APMV6 infected pool.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">These data further contribute to the knowledge about the genetic diversity within the serotypes APMV4 and 6, and confirm the limited sensitivity of the HI subtyping test. Moreover, this study demonstrates the value of a random access nucleic acid amplification method in combination with massive parallel sequencing. Using only a moderate and economical sequencing effort, the characterization and full genome sequencing of APMVs can be obtained, including the identification of viruses in mixed infections.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Rosseel</LastName><ForeName>Toon</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Operational Directorate of Virology, Veterinary and Agrochemical Research Center, Ukkel, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lambrecht</LastName><ForeName>Bénédicte</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Vandenbussche</LastName><ForeName>Frank</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>van den Berg</LastName><ForeName>Thierry</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Van Borm</LastName><ForeName>Steven</ForeName><Initials>S</Initials></Author></AuthorList><Language>eng</Language><DataBankList CompleteYN="Y"><DataBank><DataBankName>GENBANK</DataBankName><AccessionNumberList><AccessionNumber>JN571485</AccessionNumber><AccessionNumber>JN571486</AccessionNumber><AccessionNumber>JN571487</AccessionNumber><AccessionNumber>JN571488</AccessionNumber><AccessionNumber>JN571489</AccessionNumber><AccessionNumber>JN571490</AccessionNumber></AccessionNumberList></DataBank></DataBankList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2011</Year><Month>10</Month><Day>06</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Virol J</MedlineTA><NlmUniqueID>101231645</NlmUniqueID><ISSNLinking>1743-422X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012367">RNA, Viral</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018109" MajorTopicYN="N">Avulavirus</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045463" MajorTopicYN="N">Avulavirus Infections</DescriptorName><QualifierName UI="Q000662" MajorTopicYN="Y">veterinary</QualifierName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001530" MajorTopicYN="N" Type="Geographic">Belgium</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004372" MajorTopicYN="N">Ducks</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014644" MajorTopicYN="Y">Genetic Variation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016679" MajorTopicYN="Y">Genome, Viral</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005838" MajorTopicYN="N">Genotype</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D059014" MajorTopicYN="N">High-Throughput Nucleotide Sequencing</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012367" MajorTopicYN="N">RNA, Viral</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012703" MajorTopicYN="N">Serotyping</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2011</Year><Month>08</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2011</Year><Month>10</Month><Day>06</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2011</Year><Month>10</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2011</Year><Month>10</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2012</Year><Month>2</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">21978491</ArticleId><ArticleId IdType="pii">1743-422X-8-463</ArticleId><ArticleId IdType="doi">10.1186/1743-422X-8-463</ArticleId><ArticleId IdType="pmc">PMC3219605</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Vet Rec. 1984 Dec 22-29;115(25-26):653</Citation><ArticleIdList><ArticleId IdType="pubmed">6098067</ArticleId></ArticleIdList></Reference><Reference><Citation>Virus Res. 2009 Oct;145(1):80-91</Citation><ArticleIdList><ArticleId IdType="pubmed">19540277</ArticleId></ArticleIdList></Reference><Reference><Citation>Arch Virol. 1983;78(1-2):29-36</Citation><ArticleIdList><ArticleId IdType="pubmed">6651534</ArticleId></ArticleIdList></Reference><Reference><Citation>Virus Res. 2009 Jun;142(1-2):10-8</Citation><ArticleIdList><ArticleId IdType="pubmed">19185593</ArticleId></ArticleIdList></Reference><Reference><Citation>Virol J. 2008;5:124</Citation><ArticleIdList><ArticleId IdType="pubmed">18937854</ArticleId></ArticleIdList></Reference><Reference><Citation>J Avian Med Surg. 2009 Sep;23(3):205-8</Citation><ArticleIdList><ArticleId IdType="pubmed">19999764</ArticleId></ArticleIdList></Reference><Reference><Citation>Avian Dis. 1997 Apr-Jun;41(2):326-9</Citation><ArticleIdList><ArticleId IdType="pubmed">9201395</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Rev Genet. 2010 Jan;11(1):31-46</Citation><ArticleIdList><ArticleId IdType="pubmed">19997069</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Biol Evol. 2011 Oct;28(10):2731-9</Citation><ArticleIdList><ArticleId IdType="pubmed">21546353</ArticleId></ArticleIdList></Reference><Reference><Citation>Res Vet Sci. 1978 Sep;25(2):204-6</Citation><ArticleIdList><ArticleId IdType="pubmed">725336</ArticleId></ArticleIdList></Reference><Reference><Citation>Arch Virol. 2002 Jul;147(7):1287-302</Citation><ArticleIdList><ArticleId IdType="pubmed">12111409</ArticleId></ArticleIdList></Reference><Reference><Citation>Acta Virol. 1984 Mar;28(2):156-8</Citation><ArticleIdList><ArticleId IdType="pubmed">6145349</ArticleId></ArticleIdList></Reference><Reference><Citation>Avian Pathol. 2008 Aug;37(4):429-34</Citation><ArticleIdList><ArticleId IdType="pubmed">18622861</ArticleId></ArticleIdList></Reference><Reference><Citation>Virology. 1993 Apr;193(2):997-1000</Citation><ArticleIdList><ArticleId IdType="pubmed">8460504</ArticleId></ArticleIdList></Reference><Reference><Citation>Virus Genes. 2008 Dec;37(3):342-50</Citation><ArticleIdList><ArticleId IdType="pubmed">18770019</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 1998 Feb;72(2):891-9</Citation><ArticleIdList><ArticleId IdType="pubmed">9444980</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2010 Nov;84(21):11496-504</Citation><ArticleIdList><ArticleId IdType="pubmed">20702635</ArticleId></ArticleIdList></Reference><Reference><Citation>J Wildl Dis. 1995 Jan;31(1):66-9</Citation><ArticleIdList><ArticleId IdType="pubmed">7563427</ArticleId></ArticleIdList></Reference><Reference><Citation>Virus Res. 2010 Jun;150(1-2):61-72</Citation><ArticleIdList><ArticleId IdType="pubmed">20206652</ArticleId></ArticleIdList></Reference><Reference><Citation>J Gen Virol. 2001 Sep;82(Pt 9):2157-68</Citation><ArticleIdList><ArticleId IdType="pubmed">11514725</ArticleId></ArticleIdList></Reference><Reference><Citation>Vet Microbiol. 1997 Oct 31;58(1):73-8</Citation><ArticleIdList><ArticleId IdType="pubmed">9451463</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2010;5(2):e9269</Citation><ArticleIdList><ArticleId IdType="pubmed">20174645</ArticleId></ArticleIdList></Reference><Reference><Citation>Dtsch Tierarztl Wochenschr. 1991 Apr;98(4):138-41</Citation><ArticleIdList><ArticleId IdType="pubmed">1829671</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2001 Sep 25;98(20):11609-14</Citation><ArticleIdList><ArticleId IdType="pubmed">11562506</ArticleId></ArticleIdList></Reference><Reference><Citation>J Wildl Dis. 1991 Jan;27(1):123-8</Citation><ArticleIdList><ArticleId IdType="pubmed">2023311</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Genomics. 2008;9:5</Citation><ArticleIdList><ArticleId IdType="pubmed">18179705</ArticleId></ArticleIdList></Reference><Reference><Citation>Avian Dis. 1981 Apr-Jun;25(2):517-20</Citation><ArticleIdList><ArticleId IdType="pubmed">7259687</ArticleId></ArticleIdList></Reference><Reference><Citation>Avian Pathol. 1982;11(1):179-85</Citation><ArticleIdList><ArticleId IdType="pubmed">18770182</ArticleId></ArticleIdList></Reference><Reference><Citation>J Gen Virol. 1978 Feb;38(2):293-301</Citation><ArticleIdList><ArticleId IdType="pubmed">627875</ArticleId></ArticleIdList></Reference><Reference><Citation>Virus Res. 2009 Jun;142(1-2):144-53</Citation><ArticleIdList><ArticleId IdType="pubmed">19341613</ArticleId></ArticleIdList></Reference><Reference><Citation>Acta Virol. 1979 Nov;23(6):504-7</Citation><ArticleIdList><ArticleId IdType="pubmed">94770</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Cell Probes. 1991 Dec;5(6):473-81</Citation><ArticleIdList><ArticleId IdType="pubmed">1664049</ArticleId></ArticleIdList></Reference><Reference><Citation>Virus Res. 2008 Nov;137(2):189-97</Citation><ArticleIdList><ArticleId IdType="pubmed">18691616</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2005 Sep 15;437(7057):376-80</Citation><ArticleIdList><ArticleId IdType="pubmed">16056220</ArticleId></ArticleIdList></Reference><Reference><Citation>Avian Dis. 2006 Sep;50(3):386-90</Citation><ArticleIdList><ArticleId IdType="pubmed">17039838</ArticleId></ArticleIdList></Reference><Reference><Citation>Virus Res. 2008 Oct;137(1):40-8</Citation><ArticleIdList><ArticleId IdType="pubmed">18603323</ArticleId></ArticleIdList></Reference><Reference><Citation>Vet Rec. 2004 Jul 31;155(5):156</Citation><ArticleIdList><ArticleId IdType="pubmed">15338713</ArticleId></ArticleIdList></Reference><Reference><Citation>Virus Res. 2010 Apr;149(1):78-85</Citation><ArticleIdList><ArticleId IdType="pubmed">20079781</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 1997 Sep 1;25(17):3389-402</Citation><ArticleIdList><ArticleId IdType="pubmed">9254694</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Belgique</li></country></list><tree><noCountry><name sortKey="Lambrecht, Benedicte" sort="Lambrecht, Benedicte" uniqKey="Lambrecht B" first="Bénédicte" last="Lambrecht">Bénédicte Lambrecht</name><name sortKey="Van Borm, Steven" sort="Van Borm, Steven" uniqKey="Van Borm S" first="Steven" last="Van Borm">Steven Van Borm</name><name sortKey="Van Den Berg, Thierry" sort="Van Den Berg, Thierry" uniqKey="Van Den Berg T" first="Thierry" last="Van Den Berg">Thierry Van Den Berg</name><name sortKey="Vandenbussche, Frank" sort="Vandenbussche, Frank" uniqKey="Vandenbussche F" first="Frank" last="Vandenbussche">Frank Vandenbussche</name></noCountry><country name="Belgique"><noRegion><name sortKey="Rosseel, Toon" sort="Rosseel, Toon" uniqKey="Rosseel T" first="Toon" last="Rosseel">Toon Rosseel</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/GrippeBelgiqueV4/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000060 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000060 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= GrippeBelgiqueV4
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:21978491
|texte= Identification and complete genome sequencing of paramyxoviruses in mallard ducks (Anas platyrhynchos) using random access amplification and next generation sequencing technologies.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:21978491" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a GrippeBelgiqueV4
| This area was generated with Dilib version V0.6.35. |  |